Composite

Part:BBa_K1650009:Design

Designed by: Daniel Stukenberg   Group: iGEM15_Marburg   (2015-09-18)


Composite carrying the Lac-inducible CDI-operon,constitutively expressed RFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6879
    Illegal PstI site found at 1325
    Illegal PstI site found at 1510
    Illegal PstI site found at 2005
    Illegal PstI site found at 2080
    Illegal PstI site found at 3112
    Illegal PstI site found at 3259
    Illegal PstI site found at 3398
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6879
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1325
    Illegal PstI site found at 1510
    Illegal PstI site found at 2005
    Illegal PstI site found at 2080
    Illegal PstI site found at 3112
    Illegal PstI site found at 3259
    Illegal PstI site found at 3398
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6879
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6879
    Illegal PstI site found at 1325
    Illegal PstI site found at 1510
    Illegal PstI site found at 2005
    Illegal PstI site found at 2080
    Illegal PstI site found at 3112
    Illegal PstI site found at 3259
    Illegal PstI site found at 3398
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6879
    Illegal PstI site found at 1325
    Illegal PstI site found at 1510
    Illegal PstI site found at 2005
    Illegal PstI site found at 2080
    Illegal PstI site found at 3112
    Illegal PstI site found at 3259
    Illegal PstI site found at 3398
    Illegal NgoMIV site found at 3133
    Illegal NgoMIV site found at 3628
    Illegal NgoMIV site found at 5203
    Illegal NgoMIV site found at 6757
    Illegal AgeI site found at 616
    Illegal AgeI site found at 728
    Illegal AgeI site found at 1768
    Illegal AgeI site found at 2942
    Illegal AgeI site found at 2995
    Illegal AgeI site found at 4030
    Illegal AgeI site found at 4795
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2492
    Illegal BsaI site found at 7323
    Illegal BsaI site found at 7744
    Illegal BsaI.rc site found at 2000
    Illegal BsaI.rc site found at 2267
    Illegal BsaI.rc site found at 11005


Design Notes

BBa_K1650009 was toxic for cells in pSB1C3 backbone, presumably because of the high metabolic burden. In accordance with iGEM Headquarter, we submitted BBa_K1650009 in the low copy pSB4C5 backbone


Source

The CDI-operon was PCR amplified from genomic DNA of E.coli strain EC93. Other parts were taken from the registry.

References